Mucopolysaccharides from Animal Tissues

Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues.

Preparation of extracts from animal tissues.

The initial procedure in the isolation of an protein, a protein complex, or a subcellular organelle is the preparation of an extract that contains the required component in a soluble form. Indeed, when undertaking a proteomic study, the production of a suitable cellular extract is essential. Further isolation of subcellular fractions depends on the ability to rupture the animal tissues in such ...

RNA Extraction from Animal and Human\'s Cancerous Tissues: Does Tissue Matter?

The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was ...

The distribution and properties of transhydrogenase from animal tissues.

where TPN+ and TPNH represent the oxidized and reduced forms oftriphosphopyridine nucleotide and DPN+ and DPNH represent the corresponding forms of diphosphopyridine nucleotide. Further studies by Kaplan, Colowick & Neufeld (1952, 1953) showed that the reactions were brought about by hydrogen, not electron, transfer and that the enzyme occurred in some animal tissues. The purpose of the present...

Structural characterization of pharmaceutical heparins prepared from different animal tissues.

Although most pharmaceutical heparin used today is obtained from porcine intestine, heparin has historically been prepared from bovine lung and ovine intestine. There is some regulatory concern about establishing the species origin of heparin. This concern began with the outbreak of mad cow disease in the 1990s and was exacerbated during the heparin shortage in the 2000s and the heparin contami...